PHR9274

2-Mercaptoethanol

certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• MDL number: MFCD00004890
• UNSPSC Code: 41116107

Properties

• grade: certified reference material; TraceCERT^®
• Quality Level: 300
• Agency: Not traceable to any primary pharmacopeia standard
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• product line: TraceCERT^®
• shelf life: limited shelf life, expiry date on the label
• expl. lim.: 18 %
• manufacturer/tradename: Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
• concentration: 14.3 M (pure liquid)
• refractive index: n20/D 1.500 (lit.)
• bp: 157 °C (lit.)
• density: 1.114 g/mL at 25 °C (lit.)
• application(s): pharmaceutical
• storage temp.: −20°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

General description

This certified reference material (CRM) is produced and certified in accordance with ISO/IEC 17025 and ISO 17034. This CRM is traceable to primary material from an NMI, e.g. NIST or NMIJ. Certified content by quantitative NMR incl. uncertainty and expiry date are given on the certificate. Download your certificate at: http://www.sigma-aldrich.com

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Legal Information

TraceCERT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Target Organs: Liver,Heart
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 165.2 °F - closed cup
• Flash Point(C): 74 °C - closed cup