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G005669

HPLC Analysis of Aswagandha Extract on Discovery® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)
mobile phase: [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient: 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate: 1.5 mL/min
pressure: 3191 psi (220 bar)
column temp.: 27 °C
detector: UV, 227 nm
injection: 20 μL
sample: 20 μg/mL in water: acetonitrile (86:14)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Discovery C18 for the efficient separation of withania extract by the USP monograph.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Withanoside IV
    Withanoside V
    Withaferin A
    12-Deoxywithastramonolide
    Withanolide A
    Withanolide B

analytical column

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standard

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