59197
SUPELCOSIL™ ABZ+Plus (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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product name
SUPELCOSIL™ ABZ+Plus HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Agency
suitable for USP L60
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
12.0% carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.4 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
SUPELCOSIL ABZ+Plus columns offer both high deactivation and unique selectivity. Deactivated silica particles of very narrow particle size distribution ensure high efficiency with low back pressure. After bonding and endcapping reactions, the ABZ+Plus phase effectively shields unreacted silanol groups on the silica, preventing them from interacting with most analytes, and provides symmetric peaks regardless of an analyte′s functionality. The phase also allows you to use low ionic strength buffers without having to add an ion-suppressing modifier. ABZ+Plus enables you to use simple mobile phases when analyzing the most difficult compounds; acids, strongly basic compounds, and zwitterions.
suitable for L60 per USP
suitable for L60 per USP
Features and Benefits
• High efficiency for polar, nonpolar, and charged analytes
• Symmetric peaks for the most reactive compounds
• Unique selectivity for polar and charged compounds
• Symmetric peaks for the most reactive compounds
• Unique selectivity for polar and charged compounds
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Journal of chromatography. A, 797(1-2), 221-226 (1998-05-16)
A reversed-phase high-performance liquid chromatographic method with electrochemical detection has been developed for the determination of buspirone from human plasma. The separation was carried out by using a Supelcosil ABZ+ plus C18 reversed-phase column and 0.05 M potassium dihydrogenphosphate (pH
Talanta, 67(5), 933-941 (2008-10-31)
A liquid-liquid extraction method using diethyl ether as organic solvent was optimized simultaneously for five 1,4-dihydropyridines (amlodipine, nitrendipine, felodipine, lacidipine and lercanidipine) belonging to the group of calcium channel blockers. Some experimental tools such as a full factorial design, a
Journal of chromatography. A, 957(2), 173-185 (2002-07-13)
The single run analysis of 23 small peptides (principally glycyl and lysyl dipeptides) is performed by ion-pair reversed-phase liquid chromatography coupled with evaporative light scattering detection or electrospray (tandem) mass spectrometry. Several perfluorinated carboxylic acid homologues are evaluated with an
Journal of separation science, 28(17), 2350-2362 (2005-12-14)
An RPLC was developed to rapidly determine lipophilicity of neutral and basic compounds using three base deactivated RPLC stationary phases particularly designed for the analysis of basic compounds, namely, Supelcosil ABZ(+)Plus, Discovery RP Amide C16, and Zorbax Extend C18. The
Journal of pharmaceutical and biomedical analysis, 37(4), 757-762 (2005-03-31)
A highly sensitive method was developed and validated for determining the free fraction of flunarizine in human plasma. Equilibrium dialysis was used for the separation of free (unbound) drug and liquid chromatography/tandem mass spectrometry (LC-MS/MS) was used for quantitation. Post-dialysis
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