Skip to Content
Merck
All Photos(1)

Key Documents

Z380059

Sigma-Aldrich

Breathe-Easy® sealing membrane

polyurethane membrane with acrylic adhesive pre-cut to fit standard multiwell plates.

Synonym(s):

breathable sealing membrane, cell culture, microplates, microwell plates, multi-well plates, multi-well sealing film, plate sealers, plate sealing film, sealing film, tissue culture

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41122410
NACRES:
NB.22

material

rectangular transparent membrane (polyurethane)

sterility

sterile

feature

piercable

packaging

pack of 100 ea

manufacturer/tradename

Diversified Biotech BEM-1

General description

Breathe-Easy® membrane is permeable to oxygen, carbon dioxide and water vapor, creating separate, highly uniform microculture vessels from a multi-well plate.
The membrane comes as a sandwich. The bottom paper liner protects the adhesive layer, and the top carrier sheet protects the outer surface of the highly compliant membrane until it has been placed and adhered to the plate. The liners also assure sterility of each individual membrane. The adhesive seals every well (unlike molded plastic covers), permitting agitation without cross-contamination or microbial infiltration. Compatible with most caustics and organic solvents, stable from −80 to +100 °C.
Note: Use of the Brayer (Z380067 sold separately) is recommended to assure uniform adhesion and to facilitate removal of the top paper liner.

Features and Benefits

Breathe-Easy® memebrane:
  • Sterile & Non-Cytotoxic: Sterilized by gamma irradiation (free of cytotoxins)
  • Prevent Contamination: Films eliminate well-to-well contamination, microbial and viral contamination, evaporation (excluding breathable membranes) and sample spillage
  • Ideally Sized and Designed: 6" x 3.25" rectangles fit all microplates. Two scored end-tabs make film positioning and removal hassle-free
  • Thermal Stability: Designed to withstand a wide temperature range
  • Easy Specimen Retrieval: Pierceable sealing films facilitate easy removal of samples with a pipet tip

Legal Information

Breathe-Easy is a registered trademark of Diversified Biotech

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Victoria Hsiao et al.
ACS synthetic biology, 4(2), 150-161 (2014-05-23)
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this
Teng Wang et al.
Nature communications, 11(1), 5589-5589 (2020-11-06)
Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid's ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the
Adithi R Varadarajan et al.
NPJ biofilms and microbiomes, 6(1), 46-46 (2020-11-01)
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a
Nathan A Sallee et al.
SLAS discovery : advancing life sciences R & D, 25(9), 1047-1063 (2020-07-28)
The identification of novel peptide hormones by functional screening is challenging because posttranslational processing is frequently required to generate biologically active hormones from inactive precursors. We developed an approach for functional screening of novel potential hormones by expressing them in
Filipe Pinto et al.
Nature communications, 11(1), 1529-1529 (2020-04-07)
Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service