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Y1375

Millipore

YPD Broth

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Yeast Peptone Dextrose Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

form

powder

composition

Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

packaging

poly bottle of 1 kg
poly bottle of 250 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

color

beige

pH

6.3-6.7

application(s)

environmental
food and beverages
microbiology

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Related Categories

General description

YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.

Application

YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.

Quantity

50 g per liter

Preparation Note

1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yeast Extract for use in microbial growth medium

Sigma-Aldrich

Y1625

Yeast Extract

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Biotechnology for biofuels, 5(1), 49-49 (2012-07-21)
The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal cellulase, and fermentation
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Journal of the American Chemical Society, 136(11), 4333-4342 (2014-03-13)
Fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. Previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen Candida albicans
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Developmental and comparative immunology, 78, 149-159 (2017-10-08)
The nuclear factor of activated T cells (NFAT) proteins have crucial roles in the development and function of the immune system since they not only regulate activation of T cells but are also involved in the control of thymocyte development
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G3 (Bethesda, Md.), 8(5), 1637-1647 (2018-03-15)
Candida glabrata (C. glabrata) forms part of the normal human gut microbiota but can cause life-threatening invasive infections in immune-compromised individuals. C. glabrata displays high resistance to common azole antifungals, which necessitates new treatments. In this investigation, we identified five
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