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Key Documents

SRP8026

Sigma-Aldrich

Annexin V human

recombinant, expressed in E. coli, GST tagged, ≥95% (SDS-PAGE)

Synonym(s):

Annexin A5, Calphobindin I, EC 2.7.1.23, Endonexin II, Placental anticoagulantprotein I

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

tag

GST tagged

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

~52 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

activity assay: suitable

color

white

UniProt accession no.

P08758

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ANXA5(308)

General description

Annexin V is a member of the annexin family of calcium and membrane-binding proteins that have anticoagulatory and anti-inflammatory properties. The encoded protein is 320 amino acid long and is folded into four domains, each having five α-helices, forming a superhelical globular structure. The ANX5 gene is mapped to human chromosome 4q26-q28 and spans a length of 28kb containing 13 exons and 12 introns.

Application

Annexin V has been used to examine phosphorylation activity of ChaK1 (channel-kinase 1).

Biochem/physiol Actions

Annexins are a family of water-soluble proteins that bind to membranes and phospholipids in a calcium-dependent manner. They participate in several cellular functions, such as membrane fusion and cytoskeletal interaction. Annexin V is a potent anticoagulant and serves as a voltage-gated calcium channel when bound to membrane in vitro. It is found to inhibit phospholipase A2 and protein kinase C.

Physical form

Lyophilized from PBS, pH 7.4.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

Recombinant human annexin V fused to a GST-tag.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oncotarget, 8(26), 42602-42612 (2017-04-14)
Annexin V, a protein with high affinity to phosphatidylserine (PS) in a calcium dependent manner, has been widely used to probe apoptosis. Annexin V in inhibiting engulfment of apoptotic cells by macrophages had been reported to increase the immunogenicity of
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