SRP4623P

Myostatin Proform human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): GDF-8, Growth Differentiation Factor 8, MSTN, MSTN Muscle Hypertrophy
• MDL number: MFCD03457656
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~27.8 kDa
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• NCBI accession no.: O14793
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MSTN(2660)

Description

General description

MSTN (myostatin) belongs to the transforming growth factor-β (TGF β) superfamily containing numerable homologous cytokine proteins. This gene is expressed in skeletal muscle, and codes for a precursor protein composed of an N-terminal leader peptide, a pro-peptide domain, and a C-terminal domain. This precursor protein is proteolytically cleaved by bone morphogenetic protein 1 (BMP-1) at a conserved four amino acid RSRR site to produce the 13kDa mature protein.

Biochem/physiol Actions

MSTN (myostatin) functions as a negative modulator of muscle cell development. It functions through forming heteromeric receptor complexes such as with activin type II receptor B (ActRIIB), and with lesser affinity with ActIIA. This interaction results in the recruitment of type I receptors like activin receptor-like kinase 4 (ALK4) or ALK5, leading to the phosphorylation of Smad2 (small mothers against decapentaplegic) and Smad3, which move to the nucleus and suppress myogenesis. In MSTN-null mice, there is delayed skin wound healing due to the increased expression of decorin, an endogenous transforming growth factor-β (TGF β) suppressor. Topical treatment with TGF-β1 restored skin wound healing, suggesting that MSTN agonists might have therapeutic beneficial in skin wound repair.

Physical form

Lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1 to 1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable