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Key Documents

SAB4500827

Sigma-Aldrich

Anti-Factor VIII antibody produced in rabbit

affinity isolated antibody

Synonym(s):

AHF, F8B, F8C, antihemophilic factor, coagulation factor VIII

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 267 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

P00451

UniProt accession no.

P00451

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... F8(2157)

Related Categories

General description

Anti-Factor VIII Antibody detects endogenous levels of total Factor VIII protein.
Blood clotting factor VIII (FVIII), a nonenzymatic cofactor of activated factor IX (FIXa) is of ~330 kDa. It has three homologous A-domains, a unique B-domain and two C-domains. It is located on chromosome Xq28.

Immunogen

The antiserum was produced against synthesized peptide derived from human Factor VIII.

Immunogen Range: 2161-2210

Biochem/physiol Actions

Mutation in the factor VIII gene (F8) results in haemophilia A (HA). Higher levels of VIII:C (FVIII:C) leads to venous and arterial thromboembolism. The factor VIII serve as a procoagulant cofactor in the intrinsic blood coagulation pathway.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elevated levels of FVIII:C within families are associated with an increased risk for venous and arterial thrombosis
Bank I, et al.
Journal of Thrombosis and Haemostasis, 3(1), 79-84 (2005)
An in silico and in vitro approach to elucidate the impact of residues flanking the cleavage scissile bonds of FVIII
Pezeshkpoor B, et al.
PLoS ONE, 12(7) (2017)
Mutational spectrum and deep intronic variants in the factor VIII gene of haemophilia A patients. Identification by next generation sequencing
Bach JE, et al.
Hamostaseologie, 36(S 02), S25-S28 (2016)
Localization of human factor FVIII inhibitor epitopes to two polypeptide fragments
Fulcher CA, et al.
Proceedings of the National Academy of Sciences of the USA, 82(22), 7728-7732 (1985)
Mapping of X-linked myxomatous valvular dystrophy to chromosome Xq28
Kyndt F, et al.
American Journal of Human Genetics, 62(3), 627-632 (1998)

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