RAB0362

Human MMP-1 ELISA Kit

for cell and tissue lysates

• Synonym(s): MMP-1, Matrix metalloproteinase-1
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type tissue lysate; sample type cell lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 8 pg/mL; standard curve range: 24.69-18000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MMP1(4312)

Description

General description

The Human MMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-1 cell lysate and tissue lysate.

Immunogen

Recombinant Human MMP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular diseases, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with rheumatoid arthritis and osteoarthritis. MMP-1 is linked with juvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease. MMP-1 might facilitate fibrous plaque disruption via degradation of interstitial collagens and thinning of the fibrous cap.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABDIL6 ELISA 5X Sample Diluent Buffer (Item D2)
• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABLYSIS1 2X Cell Lysis Buffer (Item J)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable