PD1
PeroxiDetect™ Kit
Synonym(s):
Peroxidase assay kit
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
Recommended Products
Quality Level
storage temp.
2-8°C
General description
The PeroxiDetect Kit For the Determination of Aqueous and Lipid Hydroperoxides was developed for the measurement of peroxides in biological systems, which is an important factor in determining the degree of free radicals present in specific tissues.
Application
For the determination of aqueous peroxides and lipid hydroperoxides. Peroxides serve as a source for hydroxyl or peroxyl reactive radicals. These radicals can interact with DNA, proteins or lipid components of cells, causing cell damage that could lead to cell death. The measurement of peroxides in biological systems is an important factor in determining the degree of free radicals present in specific tissues. Lipid peroxidation has been proposed to contribute to various pathophysiological cell and tissue abnormalities. For example, increased levels of lipid peroxidation products in red blood cells were found to correlate well with the onset of diabetes mellitus in pregnant women. Cholesterol hydroperoxides accumulate in patients having excessive blood alcohol. Measurement of hydrogen peroxide in tissues has been used to study several aspects of free radical damage such as skin aging as induced by UV light and the effect of H2O2 as an inducer of elevated tyrosinase levels in melanoma cells. H2O2 has been shown to be a potent mitogen for growth-arrested cultured human aortic smooth muscle cells. The PeroxiDetect kit is capable of detection of H2O2 in aqueous solutions in the range of 1-7 nanomoles per reaction volume or of lipid hydroperoxides in organic solvents in the range of 1-16 nanomoles per reaction volume. This kit is based on the fact that peroxides will convert Fe2+ ion to Fe3+ ion at acidic pH. The Fe3+ ion will form a colored adduct with xylenol orange which is observed at 560 nm.
PeroxiDetect™ Kit is suitable for use:
- in the quantification of hydroperoxides in synaptosomal preparation
- in measuring lipid peroxidation in melanoma cells
- to quantify peroxides in the cerebral cortex
- intestinal lumen sample
Peroxides will convert Fe2+ to Fe3+ ions under acidic conditions. Fe3+ ions will then form a colored adduct with xylenol orange, which is observed at 560 nm.
Components
Kit contains reagents for 100 tests of aqueous peroxide and 100 tests of organic peroxide.
Legal Information
PeroxiDetect is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- tert-Butyl hydroperoxide 1 mL
- Hydrogen peroxide 1 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Org. Perox. C - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
5.2 - Organic peroxides and self-reacting hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J M Herbert et al.
FEBS letters, 395(1), 43-47 (1996-10-14)
Increased generation of active oxygen species such as hydrogen peroxide (H202) may be important in vascular smooth muscle cell growth associated with atherosclerosis and restenosis. In this work, we showed that H202 was a potent mitogen for growth-arrested cultured human
D Carone et al.
European journal of obstetrics, gynecology, and reproductive biology, 51(2), 103-109 (1993-10-01)
Oxygen free radicals produced during normal aerobic metabolism have been implicated in several pathophysiological mammalian processes. The importance of free radical-mediated fatty acid oxidation has received much attention. The generation of active oxygen species may lead to lipid peroxidation and
E Karg et al.
The Journal of investigative dermatology, 100(2 Suppl), 209S-213S (1993-02-01)
The effects of systems generating active oxygen species (superoxide anion, hydrogen peroxide, hydroxyl radical) on tyrosinase have been studied in cultured human melanoma cells. Tyrosinase activity was determined by measuring the quantity of 5-S-L-cysteinyl-L-dopa (5-S-CD) formed in the presence of
J Adachi et al.
Alcoholism, clinical and experimental research, 22(3 Suppl), 103S-107S (1998-06-11)
Profiles of very long-chain fatty acids were studied in the erythrocyte membrane of five alcoholic patients. We identified three fatty acids as cis-16-pentacosenoic acid (C25:1), cis-17-hexacosenoic acid (C26:1), and hexacosenoic acid (C26:1), and hexacosanoic acid (C26:0) by gas chromatography-mass spectrometry.
Formation of malondialdehyde (MDA), 4-hydroxy-2-hexenal (HHE) and 4-hydroxy-2-nonenal (HNE) in fish and fish oil during dynamic gastrointestinal in vitro digestion
Larsson K, et al.
Food & Function, 7(2), 1176-1187 (2016)
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