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Key Documents

P6186

Sigma-Aldrich

Thidiazuron

BioReagent, suitable for plant cell culture

Synonym(s):

1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea, TDZ

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About This Item

Empirical Formula (Hill Notation):
C9H8N4OS
CAS Number:
Molecular Weight:
220.25
Beilstein:
1078092
EC Number:
MDL number:
UNSPSC Code:
10171502
PubChem Substance ID:
NACRES:
NA.72

product line

BioReagent

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

SMILES string

O=C(Nc1ccccc1)Nc2cnns2

InChI

1S/C9H8N4OS/c14-9(12-8-6-10-13-15-8)11-7-4-2-1-3-5-7/h1-6H,(H2,11,12,14)

InChI key

HFCYZXMHUIHAQI-UHFFFAOYSA-N

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Application

Thidiazuron has been used:
  • as a component of the proliferation medium to test its effect on the proliferation of embryo-like structures obtained from the post-culture of shoot tips
  • as a growth regulator in Murashige and Skoog (MS) media to culture the nodal segments of Hyssopus officinalis L. seedlings
  • as a cytokinin in induction media to experiment with the hormonal composition

Biochem/physiol Actions

Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin used in micropropagations to supplement media such as Murashige and Skoog medium. TDZ promotes plant organogenesis (shoot regeneration) and plant regeneration. Thidiazuron (TDZ) enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. It is also used as a phenylurea herbicide for controlling weeds in agricultural and nonagricultural practices. Thidiazuron acts as a pesticide by inhibiting photosystem II.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wanwimon Lualon et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 96-100 (2008-04-05)
An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The
María Laura Fontana et al.
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 33(3), 179-186 (2010-01-14)
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: alpha-naphthalenacetic
Mukund Shukla et al.
Methods in molecular biology (Clifton, N.J.), 11013, 279-289 (2012-11-28)
Micropropagation is an important tool for rapid multiplication and the creation of genetic variability in African violets (Saintpaulia ionantha Wendl.). Successful in vitro propagation depends on the specific requirements and precise manipulation of various factors such as the type of
Bi-Hong Feng et al.
Journal of plant physiology, 169(2), 176-182 (2011-11-29)
Embryogenic callus (EC) induced from petioles of alfalfa (Medicago sativa L. cv. Jinnan) on B5h medium turned green, compact and non-embryogenic when the kinetin (KN) in the medium was replaced partially or completely by thidiazuron (TDZ). The application of CoCl₂
Ruphi Naz et al.
Acta biologica Hungarica, 63(4), 474-482 (2012-11-09)
An efficient and reproducible protocol for in vitro multiplication of Bauhinia tomentosa L. was developed. Multiple shoots were regenerated from cotyledonary node and stem nodal segments excised from in vitro raised seedlings on Murashige and Skoog (MS) medium supplemented with

Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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