P6110
Protease from Aspergillus oryzae
≥500 U/g
Synonym(s):
Flavourzyme®
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About This Item
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form
liquid
specific activity
≥500 U/g
storage temp.
2-8°C
General description
Proteases belong to the group of hydrolases and exist as acid, neutral, and alkaline proteases.
Fungal protease/peptidase complex produced by submerged fermentation of a selected strain of Aspergillus oryzae and contains both endoprotease and exopeptidase activities.
Application
Protease from Aspergillus oryzae has been used:
- to study the effect of protease inhibitors from Moringa oleifera
- to evaluate its hydrolyzing effect on the anatomical structure and chemical composition of archaeological wood samples
- to perform protease digestion to process mucosa samples obtained from the colon of pigs
Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. It has been injected into flies with a nanoject apparatus for infection and survival experiments.
The enzyme from Sigma has been used in the semi-purification of mouse colorectal mucins during protein digestion.
Biochem/physiol Actions
Proteases catabolize proteins by hydrolysis of peptide bonds. They have many applications such as in detergents, bioremediation processes, pharmaceutical industry, and food industries. Proteases are associated with nitrogen mineralization in the soil. They also serve as a supplement in swine and poultry diets.
Unit Definition
One unit is the amount of enzyme which hydrolyzes 1 mmol of L-Ieucine-p-nitroanilide per minute.
Legal Information
A product of Novozyme Corp.
Flavourzyme is a registered trademark of Novozymes Corp.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Too many different protein and enzyme purification techniques have been reported, especially, chromatographic techniques. Apart from low recovery, these multi-step methods are complicated, time consuming, high operating cost. So, alternative beneficially methods are still required. Since, the outstanding advantages of
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