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P4780

Sigma-Aldrich

TWEEN® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonym(s):

POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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About This Item

CAS Number:
9005-65-6
MDL number:
MFCD00082107
UNSPSC Code:
12161700
NACRES:
NA.25

description

non-ionic

form

viscous liquid

mol wt

average mol wt ~1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

CMC

0.012 mM

density

1.06 g/mL at 25 °C (lit.)
1.07 g/cm3 at 25 °C (lit.)

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used:
  • in Listeria monocytogenes enumeration using filtration method
  • as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion
  • as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

>235.4 °F - closed cup

Flash Point(C)

> 113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fats in Food Technology (2014)
A contribution to the improvement of Listeria monocytogenes enumeration in cold-smoked salmon
Besse NG, e al.
International Journal of Food Microbiology, 91(2), 119-127 (2004)
Ice Cream, 86-86 (2003)
Low-fat Gouda cheese made from bovine milk-olive oil emulsion: physicochemical and sensory attributes
Felfoul I, et al.
Journal of Food Science and Technology, 52(10), 6749-6755 (2015)
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BMC complementary and alternative medicine, 17(1), 347-347 (2017-07-05)
The essential oil from Mesosphaerum sidifolium (L'Hérit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity. EOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation
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