P1860

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

• Synonym(s): Protease Inhibitor Solution, protease inhibitor
• EC Number: 200-664-3
• MDL number: MFCD00677817
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
• Quality Level: 200
• form: DMSO solution
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Application

Protease Inhibitor Cocktail has been used:

• as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
• to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
• in trypsin solution for trypsin-inhibited studies
• as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Biochem/physiol Actions

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

Features and Benefits

• Broad specificity
• Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
• Contains no metal chelators: Ensures compatibility with downstream applications
• Convenient packaging: 1 mL in glass bottle
• Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Components

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C