N8271

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(s): Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase
• CAS Number: 9001-67-6
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• MDL number: MFCD00131711
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: buffered aqueous solution
• specific activity: ≥135 units/mg protein
• mol wt: 88 kDa; 95 kDa
• foreign activity: β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Unit Definition

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Preparation Note

Expressed in glycosidase-free hosts.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves