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MAK076

Sigma-Aldrich

Ethanol Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new Ethanol Assay Kit, MAK481, is now available! Ethanol is a psychoactive component of many commonly consumed drinks where it acts as a central nervous system depressant. After ingestion, ethanol is absorbed into the bloodstream via the stomach and small intestine. Ethanol is largely metabolized by the liver but is also secreted in urine or through respiration. The monitoring of ethanol levels is also important in fermentation processes. The ethanol assay kit provides a simple and reliable method for the quantification of ethanol.
This kit is highly sensitive to the presence of short-chain alcohols (ethanol, methanol, and propanol). Storage or use of this kit in the vicinity of alcohol vapors can result in the uptake of the alcohols by kit components, resulting in very high backgrounds.

Application

Ethanol Assay Kit has been used :
  • to measure the levels of ethanol in yeast cell culture supernatant and to study the link between metabolism and chromatin structure
  • to determine the ethanol levels in compound beverages and foods
  • to measure ethanol content in vinegar

Suitability

Suitable for determining the concentration of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.

Principle

Ethanol concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λex = 587 nm) product, proportional to the ethanol present. The Ethanol Assay Kit provides a simple and reliable method for the quantification of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste.
Roda A, et al.
Food Chemistry, 229, 734-742 (2017)
Detecting ethanol and acetaldehyde by simple and ultrasensitive fluorimetric methods in compound foods.
Zachut M, et al.
Food Chemistry, 201, 270-274 (2016)
Michael S Patton et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 46(2), 351-359 (2020-07-15)
Compulsive alcohol consumption is a core, treatment-resistant feature of alcohol use disorder. The dorsomedial and dorsolateral striatum support goal-directed and habitual action strategies, respectively. How ethanol targets dorsolateral striatum to drive compulsive consumption is poorly understood. Parvalbumin-expressing striatal fast-spiking interneurons
Reduced histone expression or defect in chromatin assembly induce respiration.
Galdieri L, et al.
Molecular and Cellular Biology (2016)
M Zachut et al.
Food chemistry, 201, 270-274 (2016-02-13)
There is a need for simple, accurate, and rapid analysis of ethanol (Eth) and acetaldehyde (AA) in a wide variety of beverages and foods. A novel enzymatic assay coupled to formation of fluorescent chromophore is presented. Eth detection was further

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