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Key Documents

IP50

Millipore

Protein G Immunoprecipitation Kit

sufficient for 50 assays

Synonym(s):

Immunoprecipitation kit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

usage

sufficient for 50 assays

technique(s)

immunoprecipitation (IP): suitable

storage temp.

2-8°C

Application

The kit is designed to allow maximal recovery of immunoprecipitates. It provides all the necessary reagents to perform immunoprecipitation from cell extracts of any protein to which a suitable antibody is available. Based on protein G, the kit binds to most commonly used antibodies. In addition, spin columns are provided to enable quick washes without the loss of protein G resin and thus protein yield is maximized.

Features and Benefits

  • Minimal loss of antigen-antibody bound beads during washing.
  • Minimal or no non-specific signals by increasing the stringency of the washing step.

Preparation Note

When preparing reagents, use ultrapure water (17M -cm)

Kit Components Also Available Separately

Product No.
Description
SDS

  • S65465 M Sodium chloride solution 15 mLSDS

  • P3296Protein G Agarose 2 mLSDS

  • 7173610% Sodium dodecyl sulfate solution 1 mLSDS

related product

Pictograms

FlameExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R Meller et al.
Cell death and differentiation, 10(5), 539-547 (2003-05-03)
Seizure-induced neuronal death may involve engagement of the BCL-2 family of apoptosis-regulating proteins. In the present study we examined the activation of proapoptotic BAD in cultured hippocampal neurons following seizures induced by removal of chronic glutamatergic transmission blockade. Kynurenic acid
Hubert Arokium et al.
The Journal of biological chemistry, 282(48), 35104-35112 (2007-10-04)
During apoptosis, the pro-apoptotic protein Bax relocalizes from the cytosol to the mitochondrial outer membrane. This relocalization is associated to major conformational changes, namely at the N- and C-terminal ends of the protein. Substitution of residues located at critical positions
F Gautier et al.
Molecular and cellular biology, 31(4), 832-844 (2010-12-22)
Bcl-2 homologues (such as Bcl-x(L)) promote survival in part through sequestration of "activator" BH3-only proteins (such as Puma), preventing them from directly activating Bax. It is thus assumed that inhibition of interactions between activators and Bcl-x(L) is a prerequisite for
Kong-Hung Sze et al.
Cell chemical biology, 24(2), 182-194 (2017-01-24)
Talaromyces (Penicillium) marneffei is one of the leading causes of systemic mycosis in immunosuppressed or AIDS patients in Southeast Asia. How this intracellular pathogen evades the host immune defense remains unclear. We provide evidence that T. marneffei depletes levels of a
Signal Transduction Protein Array Analysis Links LRRK2 to Ste20 Kinases and PKC Zeta That Modulate Neuronal Plasticity
Zach,S et al.
PLoS ONE, 5(10) (2010)

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