HPA035977

Anti-KMT2D antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): ALR, CAGL114, MLL2, MLL4, TNRC21
• MDL number: MFCD00675261
• UNSPSC Code: 12352203
• Human Protein Atlas Number: HPA035977
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• product line: Prestige Antibodies^® Powered by Atlas Antibodies
• form: buffered aqueous glycerol solution
• species reactivity: human
• enhanced validation: orthogonal RNAseq; Learn more about Antibody Enhanced Validation
• technique(s): immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:200-1:500
• UniProt accession no.: O14686
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MLL2(8085)

Description

General description

The gene KMT2D (lysine N-methyltransferase 2D) is mapped to human chromosome 12q13. It belongs to the KMT2 family of histone lysine methyltransferases. The protein has a PHD (plant homeodomain) domain, two FY-rich motifs (FYRC and FYRN) and a catalytic SET (su(var)3-9, enhancer-of-zeste and trithorax) domain.

Immunogen

Lysine (k)-specific methyltransferase 2d recombinant protein epitope signature tag (PrEST)

Sequence
AQLADTLFSKGLGPWDPPDNLAETQKPEQSSLVPGHLDQVNGQVVPEASQLSIKQEPREEPCALGAQSVKREANGEPIGAPGTSNHLLLAGPRSEAGHLLLQKLLRAKNVQLSTGRGSEGLRAEINGHI

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Anti-KMT2D antibody produced in rabbit has been used in immunohistochemistry, immunofluorescence and chromatin immunoprecipitation.

Biochem/physiol Actions

KMT2D (lysine N-methyltransferase 2D) work as a catalytic subunit for COMPASS (complex of proteins associated to Set1)-like complex and is responsible for the methylation of lysine residue in histone 3. It is required for generation of H3K4me1 (monomethylated histone H3 lysine 4). Changes in the expression of this gene modulate the outcome in pancreatic ductal adenocarcinoma. Mutations in this gene are associated with Kabuki syndrome. KMT2D is also a frequently mutated gene in diffuse large B cell lymphoma (DLBCL) and follicular lymphoma (FL). Loss of this gene enhances lymphomagenesis.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST86867

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable