H9636

Hypoxanthine

powder, suitable for cell culture, BioReagent

• Synonym(s): 6-Hydroxypurine
• Empirical Formula (Hill Notation): C₅H₄N₄O
• CAS Number: 68-94-0
• Molecular Weight: 136.11
• Beilstein: 5811
• EC Number: 200-697-3
• MDL number: MFCD00005725
• UNSPSC Code: 12352207
• PubChem Substance ID: 24895864
• NACRES: NA.75

Properties

• product name: Hypoxanthine, powder, BioReagent, suitable for cell culture
• product line: BioReagent
• Assay: ≥99%
• form: powder
• technique(s): cell culture | mammalian: suitable
• mp: >300 °C (lit.)
• solubility: formic acid: water (2:1): 50 mg/mL
• SMILES string: O=C1NC=Nc2nc[nH]c12
• InChI: 1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
• InChI key: FDGQSTZJBFJUBT-UHFFFAOYSA-N

Description

General description

Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).

Application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.

Hypoxanthine has been used:

• as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3
• in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)
• to generate superoxide anions
• to prepare hybridoma selection and maintenance media.

Biochem/physiol Actions

Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves