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H9037

Sigma-Aldrich

Anti-HA−TRITC antibody, Mouse monoclonal

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

conjugate

TRITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

buffered aqueous solution

storage condition

protect from light

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

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General description

Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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