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FLAAM

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix

lyophilized powder

Synonym(s):

ATP assay kit, ATP detection kit

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

form

lyophilized powder

usage

1 vial sufficient for ≥50 assays

technique(s)

PCR: suitable

storage temp.

−20°C

General description

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.

Components

Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.

Reconstitution

Reconstitute with 5 ml of sterile water.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anita Jemec et al.
Environment international, 87, 20-32 (2015-12-08)
Within the FP7 EU project NanoValid a consortium of six partners jointly investigated the hazard of silver nanoparticles (AgNPs) paying special attention to methodical aspects that are important for providing high-quality ecotoxicity data. Laboratories were supplied with the same original
Nazlıhan Aztopal et al.
Chemico-biological interactions, 280, 51-58 (2017-12-12)
Cancer stem-like cells (CSCs) are a cell subpopulation that can reinitiate tumors, resist chemotherapy, give rise to metastases and lead to disease relapse because of an acquired resistance to apoptosis. Especially, epigenetic alterations play a crucial role in the regulation
Nobuyuki Nishikawa et al.
Neurourology and urodynamics, 39(6), 1644-1652 (2020-06-13)
Bladder wall stretch increases tissue tension and releases adenosine 5'-triphosphate (ATP) as part of a transduction process to sense bladder filling. Aging is associated with bladder fibrosis to produce a stiffer bladder wall: this may augment ATP release and contribute
Ann K Rosenthal et al.
Arthritis research & therapy, 15(5), R154-R154 (2013-11-30)
Extracellular ATP (eATP) is released by articular chondrocytes under physiological and pathological conditions. High eATP levels cause pathologic calcification, damage cartilage, and mediate pain. We recently showed that stable over-expression of the progressive ankylosis gene product, ANK, increased chondrocyte eATP
Buse Cevatemre et al.
Chemico-biological interactions, 260, 248-255 (2016-10-11)
Despite the rapid advances in chemotherapy regimens, the outcome of patients with breast cancer is not satisfactory. One of the reasons of this dissatisfaction is that subsets of cells in tumors which referred as cancer stem cells (CSCs) show and/or

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