Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.[1][2]
Reconstitution
Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.
Reproductive biology and endocrinology : RB&E, 10, 102-102 (2012-12-04)
Since Xenarthra are serious candidates for being basal to Eutheria, their characteristics, e.g. the placental system, influence perceptions of evolution. However, in the subgroup containing the anteaters, data are very limited. The present study aims to elucidate the nature of
British journal of cancer, 80(3-4), 546-555 (1999-07-17)
The molecular mechanisms leading to androgen-independent growth in prostate cancer (PC) are poorly understood. Androgen deprivation therapy (ADT) results physiologically in a decrease in proliferation and an increase in programmed cell death (PCD)/apoptosis. The aim of our study was to
Histochemical studies of enzyme activities and structural elements in Gyrodactylus derjavini Mikailov, 1975 parasitizing fins of Oncorhynchus mykiss Walbaum were conducted. Marked activities of non-specific esterase, acid phosphatase, alkaline phosphatase and amino-peptidase were found in the intestinal caeca of the
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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