Skip to Content
Merck
All Photos(4)

Key Documents

E1894

Sigma-Aldrich

EPPS

BioXtra, ≥99.5% (titration)

Synonym(s):

Triethanolamine hydrochloric acid salt, 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C9H20N2O4S
CAS Number:
Molecular Weight:
252.33
Beilstein:
3957385
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product line

BioXtra

Quality Level

Assay

≥99.5% (titration)

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

5.0-7.0 (20 °C, 1 M in H2O)

useful pH range

7.3-8.7

pKa 

8

mp

237-239 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

bromide (Br-): ≤0.1%

cation traces

Al: ≤0.0005%
Ca: ≤0.002%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.1%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.1 at 260 in H2O at 1 M
≤0.1 at 280 in H2O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCCS(O)(=O)=O)CC1

InChI

1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)

InChI key

OWXMKDGYPWMGEB-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

EPPS, also known as 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid, is a zwitterionic buffering agent that belongs to the group of good buffers. It plays a vital role in maintaining a stable pH in solutions, which is critical for various biological and biochemical processes. EPPS buffer finds extensive application in biological research and cell culture experiments.

Application

EPPS buffer has been used as a component of buffer solution for methylglyoxal (MG) treatment in vitro to form advanced glycation end products (AGEs) to crosslink collagen fibrils. It has also been used in the preparation of fixation buffer solution for light microscopy.

Features and Benefits

  • The BioXtra product line ensures T15 high-quality products for consistent results.
  • A high assay of ≥99.5% guarantees the purity of the product.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Equilibrium studies, synthesis, and characterization of zwitterionic buffer (HEPES and HEPPS) dimethyltin (IV) complexes
Mahmoud M, et al.
Main Group Chemistry, 6(2) (2007)
Equalization of Cleavage Is Not Causally Responsible for Specification of Cell Lineage
Edgar A, et al.
The Biological Bulletin, 237(3), 250-253 (2019)
lonization Enthalpies of Some Common Zvvitterionic Hydrogen-lon Buffers (HEPES, PIPES, HEPPS and BES) for Biological Research
Roig T, et al.
Journal of Separation Science, 993(47), 899-90j-899-90j (1993)
Allison Edgar
The Biological bulletin, 237(3), 250-253 (2020-01-11)
An unequal cleavage gives rise to a dedicated population of larval skeletogenic cells in sea urchins. The timing of this unequal cleavage, associated localization of key lineage markers, and loss of this lineage when embryos are treated with cleavage-equalizing reagents
Methylglyoxal crosslinking increases the fracture toughness of gelatin hydrogels
Shrivastava A, et al.
Applied Mechanics and Materials, 176, 104524-104524 (2023)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service