Vero/hSLAM

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 4091501, African green monkey kidney, Fibroblast

• Synonym(s): Vero/hSLAM Cells
• UNSPSC Code: 41106514

Properties

• biological source: African green monkey kidney
• description: Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• packaging: tube of 5 μg 04091501-DNA-5UG; pkg of vial of cells 04091501-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: SLAM; also known as CDw150, a receptor for measles virus
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Monkey African Green Kidney human CDw150 (SLAM) mealses virus receptor expression

Cell Line Description

Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which is a receptor for measles virus. Clones of transfected cells were selected in the presence of geneticin (also known as G418). Vero/hSLAM cells can be used to isolate measles virus from human clinical samples and are a substitute for the B95a cell line for this purpose. Mumps susceptibility has also been demonstrated at the depositor′s laboratory.

Culture Medium

MEM with Earle's salts + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) + 0.4 mg/ml Geneticin. It is essential that geneticin is added to the culture medium as it is required to maintain the expression of the SLAM gene.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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