A9758

ACES

≥99.0% (titration)

• Synonym(s): 2-[(2-Amino-2-oxoethyl)azaniumyl]ethanesulfonate, 2-[(Carbamoylmethyl)amino]ethane-1-sulfonic acid, N-(2-Acetamido)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)taurine
• Linear Formula: H₂NCOCH₂NHCH₂CH₂SO₃H
• CAS Number: 7365-82-4
• Molecular Weight: 182.20
• Beilstein: 2253770
• EC Number: 230-908-4
• MDL number: MFCD00008030
• UNSPSC Code: 12161700
• PubChem Substance ID: 24891495
• NACRES: NA.25

Properties

• Quality Level: 200
• Assay: ≥99.0% (titration)
• form: crystalline powder
• pH: 3.0-4.5
• useful pH range: 6.1-7.5
• pKa (25 °C): 6.8
• mp: >220 °C (dec.) (lit.)
• solubility: water: 0.523 g/mL, clear, colorless
• application(s): diagnostic assay manufacturing
• storage temp.: room temp
• SMILES string: NC(=O)CNCCS(O)(=O)=O
• InChI: 1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10)
• InChI key: DBXNUXBLKRLWFA-UHFFFAOYSA-N

Description

General description

N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES) is a zwitterionic buffer used in biochemistry and molecular biology research. It is one of the Good′s buffers with a pH range of 6.15 - 8.35. The preferred range of ACES buffer in an aqueous solution is 6.1 - 7.5.

Application

ACES has been used:

• as a component of buffered charcoal yeast extract (BYCE) agar for plating bacteria culture and Buffered Yeast Extract (BYE) liquid medium to culture bacteria
• to study the impact of the pH shift on the thermal inactivation of bacterial (Bacillus subtills) spores
• as a buffer system to study the depolymerization of starch by α-amylase during exposure to hydrostatic pressure.

It is also suitable for use: as buffers for both agarose and polyacrylamide gel electrophoresis; in the isoelectric focusing of proteins; in the analysis of bacterial autolysins in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system.

Other Notes

The product is intended for laboratory use only, and it is not suitable for drug, household, or other purposes.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves