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Key Documents

A8549

Sigma-Aldrich

Albumin, biotin labeled bovine

lyophilized powder

Synonym(s):

Biotin labeled albumin

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
34058011
NACRES:
NA.25

biological source

bovine

Quality Level

Assay

≥80% protein basis (biuret)

form

lyophilized powder

extent of labeling

8-16 mol biotin per mol albumin

technique(s)

bioconjugation: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Application

Biotin labeled bovine albumin has been used as a component in myosin V processivity experiments. It has also been used to provide biotin tags for neutravidin coating and also to suppress nonspecific cell adhesion during preparation of tension gauge tether (TGT) surface.
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues. In spleen, biotin-BSA binds to red pulp sinuses, but not white pulp vessels (including the central arteriole) nor the marginal sinus. In the thymus, prominent binding was restricted to the capillaries of the corticomedullary area.

Physical form

Contains sodium citrate buffer salts

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Taikopaul Kaneko et al.
Science advances, 6(4), eaax7413-eaax7413 (2020-02-06)
Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin.
Integrin Molecular Tension within Motile Focal Adhesions
Wang X, et al.
Biophysical Journal, 109, 2259-2267 (2019)
Florian Kulzer et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 9(12), 1761-1766 (2008-07-31)
We use photothermal microscopy to detect and image individual gold nanoparticles that are either embedded in a polymer film or immobilized in an aqueous environment. Reducing the numerical aperture of the detection optics allows us to achieve a 200-fold-enlarged detection
Tilting and Wobble of Myosin V by High-Speed Single-Molecule Polarized Fluorescence Microscopy
Beausang JF, et al.
Biophysical Journal, 104, 1263-1273 (2019)
Parastoo Maleki et al.
Molecular genetics and genomics : MGG, 292(3), 483-498 (2017-02-06)
In this article, we summarize the knowledge and best practices learned from bulk and single-molecule measurements to address some of the frequently experienced difficulties in single-molecule Förster resonance energy transfer (smFRET) measurements on G-quadruplex (GQ) structures. The number of studies

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