A5955

Antibiotic Antimycotic Solution (100×), Stabilized

suspension, suitable for cell culture, BioReagent

• Synonym(s): Penicillin-Streptomycin-Amphotericin B Suspension
• MDL number: MFCD00130520
• UNSPSC Code: 12352207
• NACRES: NA.76

Properties

• product name: Antibiotic Antimycotic Solution (100×), Stabilized, with 10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, 0.1 μm filtered, BioReagent, suitable for cell culture
• Quality Level: 200
• sterility: 0.1 μm filtered
• product line: BioReagent
• form: suspension
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: yellow
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria; fungi; yeast
• application(s): agriculture
• Mode of action: cell wall synthesis | interferes
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Antibiotic Antimycotic Solution is formulated to contain 10,000 units penicillin, 10 mg streptomycin and 25μg Amphotericin B per mL, and is solubilized in a proprietary citrate buffer. It is recommended for use in cell culture applications at 10mL/L. Antibioticantimycotic solution is generally used to prevent the growth of harmful microorganisms including bacteria and fungi in cell culture media.

Application

Antibiotic antimycotic solution has been used:

• as a supplement in different types of cell culture media.
• as a component of phosphate buffer saline (PBS) sterile solution to prevent contamination of periodontal ligament fibroblasts (PDLF) tissues that are isolated and cultured in the experiments.
• as a component of storage solution for preserving tracheal segments harvested for subsequent experimental investigation.

Biochem/physiol Actions

Mode of Action: Penicillin acts by inhibiting bacterial cell-wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out.

Antimicrobial spectrum: Includes Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable