A0466

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin

≥90% (HPLC), powder

• Synonym(s): Ac-DEVD-AFC
• Empirical Formula (Hill Notation): C₃₀H₃₄F₃N₅O₁₃
• CAS Number: 201608-14-2
• Molecular Weight: 729.61
• MDL number: MFCD01310970
• UNSPSC Code: 12352209
• PubChem Substance ID: 329770614
• NACRES: NA.32

Properties

• Quality Level: 200
• Assay: ≥90% (HPLC)
• form: powder
• color: white
• solubility: H₂O: 1 mg/mL
• storage temp.: −20°C
• SMILES string: CC(C)[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(C)=O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc2c(OC(=O)C=C2C(F)(F)F)c1
• InChI: 1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)(H,38,47)(H,40,41)(H,42,43)(H,44,45)/t17-,18-,19-,25-/m0/s1
• InChI key: GZDRODOYEFEHGG-NUDCOPPTSA-N
• Gene Information: human ... CASP3(836)

Description

Amino Acid Sequence

NAc-Asp-Glu-Val-Asp-AFC

Biochem/physiol Actions

A fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. The 7-amido-4-trifluoromethylcoumarin derivative has better membrane permeability than the 7-amido-4-methylcoumarin derivative (A1086).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves