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60218

Sigma-Aldrich

Potassium phosphate monobasic

BioUltra, for molecular biology, anhydrous, ≥99.5% (T)

Synonym(s):

Monopotassium phosphate, Potassium dihydrogen phosphate, prim.-Potassium phosphate

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About This Item

Linear Formula:
KH2PO4
CAS Number:
Molecular Weight:
136.09
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

product line

BioUltra

Assay

≥99.5% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

4.0-4.5 (25 °C, 1 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

mp

252.6 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

2.338 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.050
λ: 280 nm Amax: 0.045

SMILES string

[K+].OP(O)([O-])=O

InChI

1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

GNSKLFRGEWLPPA-UHFFFAOYSA-M

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Application

Potassium phosphate monobasic and dibasic, are used to create phosphate buffered solutions such as potassium phosphate buffered water and saline solutions used in biological applications.

Other Notes

Component of PBS - in the isolation and analysis of nuclear RNA

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isolation and analysis of nuclear RNA.
J R Nevins
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