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D2535

Sigma-Aldrich

Direct Blue 15

suitable for Histopaque® system, Powder

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About This Item

Linear Formula:
C34H24N6O16S4Na4
CAS Number:
2429-74-5
Molecular Weight:
992.80
Colour Index Number:
24400
EC Number:
219-385-3
MDL number:
MFCD00036441
UNSPSC Code:
12171500
PubChem Substance ID:
24893697
NACRES:
NA.47

product name

Direct Blue 15, suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

description

suitable for Histopaque® system

Quality Level

200

form

powder

composition

Dye content, ~44%

color

dark blue

solubility

water: 10 mg/mL, clear, blue

suitability

suitable for viability studies of collagenase-treated rat liver cells

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].COc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(OC)c4

InChI

1S/C34H28N6O16S4.4Na/c1-55-25-9-15(3-5-23(25)37-39-31-27(59(49,50)51)11-17-7-19(57(43,44)45)13-21(35)29(17)33(31)41)16-4-6-24(26(10-16)56-2)38-40-32-28(60(52,53)54)12-18-8-20(58(46,47)48)14-22(36)30(18)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

OLSOUGWNONTDCK-UHFFFAOYSA-J

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Application

May be used as a substitute for trypan blue for some biological and staining applications.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
015F00601

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CI Direct Blue 15.
IARC monographs on the evaluation of carcinogenic risks to humans, 57, 235-245 (1993-01-01)
Sarah Falk et al.
Neuroscience letters, 450(3), 229-234 (2008-12-02)
The constant cyclic respiratory activity in the brainstem requires an un-interrupted blood flow providing glucose and O(2) to neurons generating respiratory rhythm. Here we used a combination of classical vascular visualization techniques, and calcium imaging, to compare the microvascular structure
M C Bowman et al.
Journal of analytical toxicology, 6(4), 164-174 (1982-07-01)
Absorption, metabolism and tissue distribution studies were conducted in the rat with 14C-biphenyl ring-labeled Direct Blue 15, a 3,3'-dimethoxybenzidine (DiMxBzd) based azo dye; Direct Red 2, based on 3,3'-dimethylbenzidine (DiMeBzd) and corresponding benzidine congener amines. Single oral doses of the
C R Nony et al.
Journal of analytical toxicology, 7(1), 40-48 (1983-01-01)
Specific, precise and sensitive methods are described for determining traces of potentially carcinogenic metabolites of Direct Red 2 and Direct Blue 15 in rat and hamster urine and to monitor the urine from workers who may be occupationally exposed to
Koel Kumar et al.
Bioresource technology, 98(16), 3168-3171 (2007-02-27)
Studies were carried out on decolorisation and biotransformation of the dye Direct Blue-15 into 3,3'-dimethoxybenzidine (O'-dianisidine) and a sulphonated derivative by a five-member bacterial consortium. Chromatographic studies revealed further complete biodegradation of 3,3'-dimethoxybenzidine coupled with release of ammonia, but the
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