11582950001
Roche
BM Chemiluminescence ELISA Substrate (POD)
Synonym(s):
ELISA substrate
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About This Item
UNSPSC Code:
12352204
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form
solution
Quality Level
packaging
pkg of 250 mL (for 2500 wells or 1000 tubes)
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
technique(s)
ELISA: suitable
color
clear colorless
solubility
water: miscible
suitability
suitable for chemiluminescence
shipped in
wet ice
storage temp.
2-8°C
General description
The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.
Application
The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.
Features and Benefits
- Improved sensitivity
- Large dynamic range
- Rapid and constant signal
Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H2O2)
Principle
Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.
Preparation Note
Working solution: Preparation of working solution
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:
- Add to 100 parts of solution A one part of solution B.
- Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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