MAB13431

Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2

clone A-Gel VC2, Chemicon^®, from mouse

• Synonym(s): Gelatinase A, 72 kDa Type IV Collagenase
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: A-Gel VC2, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• isotype: IgG1
• suitability: not suitable for Western blot
• NCBI accession no.: NM_004530.2
• UniProt accession no.: P08253
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MMP2(4313)

Description

General description

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

Specificity

MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic.

Immunogen

Epitope: pro and active form

Human APMA-activated native 72kDa Gelatinase A.

Application

Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).

Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

ELISA (not suitable for coating, contains BSA) 1:200-1:2000

Not suitable for Western blotting


Optimal working dilution should be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Linkage

Replaces: 04-1048

Physical form

10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide

Format: Purified

Storage and Stability

Antibody is stable for 12 months when stored at 2-8ºC

Analysis Note

Control
POSTIVE CONTROL:

Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable