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CP01

Sigma-Aldrich

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

JLA20, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

General description

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876

    • Immunogen

    Chicken
    chicken cytoskeletal proteins

    Application

    Immunoblotting (1:5000-1:20000)

    Immunofluorescence (1:10000, see application references)



    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

    Warning

    Toxicity: Multiple Toxicity Values, refer to MSDS (O)

    Physical form

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    Reconstitution

    Following initial thaw, aliquot ascites and freeze (-20°C).

    Analysis Note

    Positive Control
    All animal cell lines

    Other Notes

    Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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