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Key Documents

AB10145

Sigma-Aldrich

Anti-GFP Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

GenBank accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Specificity

The antibody reacts with the 28 kDa GFP protein.

Immunogen

Synthetic peptide corresponding to the full length of GFP protein.

Application

Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
This Anti-GFP Antibody is validated for use in WB for the detection of GFP.
Western blot: 0.1 μg/mL on GFP recombinant protein.

Optimal working dilutions must be determined by the end user.

Target description

28 kDa

Physical form

Antigen Affinity Purified
Purified rabbit immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hongliang Zhang et al.
Nature communications, 13(1), 4009-4009 (2022-07-12)
Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target of diphtheria toxin. Diphthamide biosynthesis depends on the 4Fe-4S-cluster protein Dph1 catalyzing the first committed step, as well as Dph2 to Dph7
Tamás Wilheim et al.
Brain structure & function, 226(7), 2387-2399 (2021-07-16)
The endocannabinoids have been shown to target the afferents of hypothalamic neurons via cannabinoid 1 receptor (CB1) and thereby to influence their excitability at various physiological and/or pathological processes. Kisspeptin (KP) neurons form afferents of multiple neuroendocrine cells and influence
Maniraj Bhagawati et al.
Molecular biology of the cell, 32(20), br1-br1 (2021-08-05)
The outer membrane translocase (TOM) is the import channel for nuclear-encoded mitochondrial proteins. The general import pore contains Tom40, Tom22, Tom5, Tom6, and Tom7. Precursor proteins are bound by the (peripheral) receptor proteins Tom20, Tom22, and Tom70 before being imported
Pablo Garcia-Miranda et al.
Journal of virology, 90(15), 6906-6917 (2016-05-20)
Human immunodeficiency virus (HIV) replication is strongly dependent upon a programmed ribosomal frameshift. Here we investigate the relationships between the thermodynamic stability of the HIV type 1 (HIV-1) RNA frameshift site stem-loop, frameshift efficiency, and infectivity, using pseudotyped HIV-1 and
Imre Kalló et al.
Brain structure & function, 227(3), 1083-1098 (2022-01-15)
Orexin neurons are involved in homeostatic regulatory processes, including arousal and feeding, and provide a major input from the hypothalamus to the ventral tegmental area (VTA) of the midbrain. VTA neurons are a central hub processing reward and motivation and

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