07-452
Anti-dimethyl-Histone H3 (Lys27) Antibody
Upstate®, from rabbit
Synonym(s):
H3K27me2, Histone H3 (di methyl K27)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
cell based assay: suitable
dot blot: suitable
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
dimethylation (Lys27)
Gene Information
human ... H3C1(8350)
General description
Anti-dimethyl-Histone H3 (Lys27)
Specificity
Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).
Immunogen
KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3
Application
Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).
Quality
Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.
Target description
17 kDa
Physical form
Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified
Storage and Stability
Stable for 2 years at 2-8°C from date of shipment.
Analysis Note
Control
HeLa whole cell lysate, U2OS cell lysate
HeLa whole cell lysate, U2OS cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5.
Xu, CR; Schaffer, L; Head, SR; Feeney, AJ
Proceedings of the National Academy of Sciences of the USA null
Elian Perruc et al.
The Plant journal : for cell and molecular biology, 52(5), 927-936 (2007-09-26)
Embryogenesis in Arabidopsis results in mature, osmotolerant embryos within dry seeds. Late-embryogenesis programs involve the transcription factors ABI3 and ABI5, which are necessary for osmotolerance. ABI3 and ABI5 are degraded in seeds initiating germination, abolishing their protected state. However, during
Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes.
Silva, Jose, et al.
Developmental Cell, 4, 481-495 (2003)
Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response.
Wen, H; Dou, Y; Hogaboam, CM; Kunkel, SL
Blood null
Kohta Ikegami et al.
Genes to cells : devoted to molecular & cellular mechanisms, 12(1), 1-11 (2007-01-11)
In the mammalian genome, numerous CpG-rich loci define tissue-dependent and differentially methylated regions (T-DMRs). Euchromatin from different cell types differs in terms of its tissue-specific DNA methylation profile as defined by these T-DMRs. G9a is a euchromatin-localized histone methyltransferase (HMT)
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