07-414
Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
serum, Upstate®
Synonym(s):
Dimethyl-Arginine Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).
Specificity
Reactivity with other species has not been confirmed.
Recognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated ‘backbone’ peptide or an identical peptide containing symmetrically dimethylated arginines.
Immunogen
KLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G in which R* denotes asymmetric dimethylation of arginine).
Application
Detect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB.
Quality
Routinely evaluated by Western Blot HeLa nuclear extract and p110, p75, and Sam68 in RIPA lysates from Jukat cells.
Western Blot Analysis:
A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.
Western Blot Analysis:
A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.
Target description
Varies. Antibody detects proteins containing asymmetrical dimethylated arginines.
Physical form
Rabbit antiserum diluted in storage buffer (PBS, 0.035% sodium azide and 30% glycerol). Store at -20°C.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Suppression of PRMT6-mediated arginine methylation of p16 protein potentiates its ability to arrest A549 cell proliferation.
Xiuli Wang,Ying Huang,Jing Zhao,Yu Zhang,Jun Lu,Baiqu Huang
The International Journal of Biochemistry & Cell Biology null
Critical role for arginine methylation in adenovirus-infected cells.
Iacovides, DC; O'Shea, CC; Oses-Prieto, J; Burlingame, A; McCormick, F
Journal of virology null
Jeffrey K Bailey et al.
Nucleic acids research, 45(18), 10649-10671 (2017-10-05)
Antisense oligonucleotides (ASOs) are versatile tools that can regulate multiple steps of RNA biogenesis in cells and living organisms. Significant improvements in delivery, potency, and stability have been achieved through modifications within the oligonucleotide backbone, sugar and heterocycles. However, these
A proteomic analysis of arginine methylated protein complexes
Boisvert, F. M., et al
Molecular and Cellular Proteomics, 2, 1319-1330 (2003)
Elisabeth Kowenz-Leutz et al.
The EMBO journal, 29(6), 1105-1115 (2010-01-30)
Cellular signalling cascades regulate the activity of transcription factors that convert extracellular information into gene regulation. C/EBPbeta is a ras/MAPkinase signal-sensitive transcription factor that regulates genes involved in metabolism, proliferation, differentiation, immunity, senescence, and tumourigenesis. The protein arginine methyltransferase 4
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