800811O

18:1 DG

Avanti Research^™ - A Croda Brand

• Synonym(s): 1-2-di-(9Z-octadecenoyl)-sn-glycerol; DG(18:1(9Z)/18:1(9Z)/0:0); DOG
• Empirical Formula (Hill Notation): C₃₉H₇₂O₅
• CAS Number: 24529-88-2
• Molecular Weight: 620.99
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• description: 1-2-dioleoyl-sn-glycerol, neat oil
• Assay: >99% (TLC)
• form: liquid
• packaging: pkg of 1 × 10 mg (with screw cap (800811O-10mg)); pkg of 2 × 100 mg (with screw cap (800811O-200mg)); pkg of 5 × 100 mg (with screw cap 800811O-500mg); pkg of 1 × 25 mg (with screw cap (800811O-25mg))
• manufacturer/tradename: Avanti Research^™ - A Croda Brand
• lipid type: neutral glycerides; neutral lipids
• shipped in: dry ice
• storage temp.: −20°C
• InChI: 1S/C39H72O5/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-31-33-38(41)43-36-37(35-40)44-39(42)34-32-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h17-20,37,40H,3-16,21-36H2,1-2H3/b19-17+,20-18-/t37-/m0/s1
• InChI key: AFSHUZFNMVJNKX-CSWDEDFYSA-N

Description

General description

Diacylglycerol/diglyceride (DAG) is a glycerolipid, that is located at the plasma membrane. It is made up of two fatty acid chains that are covalently attached to a single glycerol molecule with the help of an ester linkage.

Application

18:1 DG has been used as a diacyl-glycerol source in diacylglycerol O-acyltransferase 1 (DGAT1) assay. It has also been used as substrate to assess compounds as potential inhibitors of DGAT-1 in DGAT-1 enzyme assay. It is suitable to use in lipid-protein overlay screen assay with the recombinant protein His-AtROP6.

Packaging

5 mL PTFE Vial with Screw Cap (800811O-10mg)

5 mL PTFE Vial with Screw Cap (800811O-200mg)

5 mL PTFE Vial with Screw Cap (800811O-25mg)

5 mL PTFE Vial with Screw Cap (800811O-500mg)

Storage and Stability

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

Other Notes

Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.

Effective concentration:
Most biological responses saturate at 20 to 250 μM sn-1,2-dioctanoylglycerol. Only sn-1,2 isomers appear to be active.

Precaution: Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable