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378011

Sigma-Aldrich

Basic Blue 3

Dye content 25 %

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About This Item

Empirical Formula (Hill Notation):
C20H26ClN3O
CAS Number:
33203-82-6
Molecular Weight:
359.89
Colour Index Number:
51004
EC Number:
251-403-5
MDL number:
MFCD00011928
UNSPSC Code:
12171500
PubChem Substance ID:
24863537
NACRES:
NA.47

form

powder or crystals

composition

Dye content, 25%

mp

205 °C (dec.) (lit.)

λmax

654 nm

ε (extinction coefficient)

≥12000 at 257-263 nm in water at 0.01 g/L
≥4500 at 289-295 nm in water at 0.01 g/L
≥45000 at 651-657 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCN(CC)c1ccc2nc3ccc(cc3[o+]c2c1)N(CC)CC

InChI

1S/C20H26N3O.ClH/c1-5-22(6-2)15-9-11-17-19(13-15)24-20-14-16(23(7-3)8-4)10-12-18(20)21-17;/h9-14H,5-8H2,1-4H3;1H/q+1;/p-1

InChI key

IURGIPVDZKDLIX-UHFFFAOYSA-M

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General description

Basic blue 3 (BB3) can be removed from aqueous solutions with the help of activated carbon prepared from rubber seed coat.
Basic blue 3 (C.I. 51104) is a cationic dye. It is generally positively charged in an aqueous solution.

Application

Basic blue 3 has a brilliant blue shade and thus has been used as a colorant in dyeing acrylic fabrics.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

861030

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H M Shapiro
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The addition of RNA content estimation to flow cytometric measurement of DNA content provides valuable information concerning cells' transitions between quiescent and proliferative states. Equilibrium staining methods employing acridine orange have been used for DNA/RNA content measurement but are difficult
Miklós Kubinyi et al.
Applied spectroscopy, 59(1), 134-139 (2005-02-22)
It has been established by combined absorption and fluorescence measurements that the cationic dye Oxazine 1 (OX) and the polyvalent anionic host calix[8]arenesulfonate (SCA8) form two complexes in simultaneous reactions: OX + SCA8 <--> OX.SCA8 (1), and OX.SCA8 + OX
A Ghanadzadeh Gilani et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 79(1), 148-155 (2011-03-29)
The absorption and fluorescence spectra of a laser dye, oxazine-1 (OX1), in liquid and liquid crystalline media were studied at room temperature. The solvatochromic method was used to determine the ground- and excited-state dipole moments by means of Lippert-Mataga, Bakshiev
Graciela P Zanini et al.
Chemosphere, 63(3), 430-439 (2005-11-26)
The binding between an oxazine dye and a humic acid was studied in aqueous solutions in the pH range 4-10 and in the supporting electrolyte (KCl) range 0.001-0.1M. A rather simple spectrophotometric method was developed to construct binding isotherms under
Markus Braun et al.
The journal of physical chemistry. A, 110(32), 9793-9800 (2006-08-11)
Wave packet motion in the laser dye oxazine 1 in methanol is investigated by spectrally resolved transient absorption spectroscopy. The spectral range of 600-690 nm was accessible by amplified broadband probe pulses covering the overlap region of ground-state bleach and
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