T6414
Trypsin inhibitor from Glycine max (soybean)
solution, sterile-filtered, suitable for, suitable for cell culture
Synonym(s):
SBTI
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
Glycine max (soybean)
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
20,100 Da
concentration
10 mg/mL
technique(s)
cell culture | mammalian: suitable
solubility
balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble
suitability
suitable for
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
Glycine max (soybean) ... KTI3(547831)
Looking for similar products? Visit Product Comparison Guide
Application
Optimized for passage of endothelial cell cultures.
Trypsin inhibitor from Glycine max (soybean) was used for the following applications:
Trypsin inhibitor from Glycine max (soybean) was used for the following applications:
- Filteration and seeding of dispersed acini
- Relative quantification of phospho-tau and immunoblotting analysis
- Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Other Notes
Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.
View more information on Trypsin Inhibitor.
Unit Definition
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
Preparation Note
Prepared in Dulbecco′s Phosphate Buffered Saline without calcium and magnesium.
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Zhiyong Mi et al.
Cancers, 15(12) (2023-06-28)
Cancer progression depends on an accumulation of metastasis-supporting physiological changes, which are regulated by cell-signaling molecules. In this regard, a disintegrin and metalloproteinase 8 (Adam8) is a transmembrane glycoprotein that is selectively expressed and induced by a variety of inflammatory
Doubly Phosphorylated Peptide Vaccines to Protect Transgenic P301S Mice against Alzheimer's Disease Like Tau Aggregation.
Monique Richter et al.
Vaccine, 2(3), 601-623 (2014)
ZhuQing Yuan et al.
Journal of immunology (Baltimore, Md. : 1950), 182(2), 811-819 (2009-01-07)
The possibility that mycobacterial infections induce variant cytokine mRNA encoding a functionally distinct protein for immune regulation has not been addressed. In this study, we reported that Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques induced expression of variant IL-4
Johann Gout et al.
Journal of visualized experiments : JoVE, (78)(78), doi:10-doi:10 (2013-08-28)
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single
Neha Phani Bhushan et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 111(2), 343-353 (2022-09-03)
Ligand targeted therapy (LTT) is a precision medicine strategy that can selectively target diseased cells while minimizing off-target effects on healthy cells. Integrin-targeted LTT has been developed recently for angiogenesis-related diseases. However, the clinical success is based on the optimal
Chromatograms
application for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service