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T1076

Sigma-Aldrich

Anti-TAP antibody, Mouse monoclonal

clone 53H8, purified from hybridoma cell culture

Synonym(s):

Anti-TIP-associated protein

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

53H8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~66 kDa

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NXF1(10482)

General description

Monoclonal Anti-TAP (mouse IgG1 isotype) is derived from the 53H8 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from Balb/c mice immunized with TAP.
TIP-associated protein (TAP), also known as nuclear export factor (NXF1), is encoded by the gene mapped to human chromosome 11q12.3. The encoded protein is a member of multigene family of evolutionarily conserved proteins. TAP contains non-canonical RNP-type RNA binding domain, four leucine-rich repeats and an NTF2-like domain. It also contains an ubiquitin-associated domain involved in the interaction with nucleoporins.

Specificity

Monoclonal Anti-TAP recognizes human TAP (~66 kDa).

Immunogen

TAP (Tip Associated Protein).

Application

Anti-TAP antibody, mouse monoclonal may be used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

TIP-associated protein (TAP) plays an important role in carrying mRNAs from the nucleus. Elevated expression of TAP or of SRp20, enhance the production of gammaretroviral Gag polyprotein. The encoded protein might facilitate the repression of two mutations caused by insertions of endogenous retroviruses into introns of cellular genes by regulating their mature transcript levels ∼2 fold.
Transporter associated with antigen processing (TAP) interacts with the RNA-binding protein Y14 protein, a participant of the mRNA-protein complex (mRNP) near the exon-exon junction of the spliced mRNAs. Formation of the TAP/p15 heterodimer is required for efficient nuclear pore complex binding and significantly enhances the ability of TAP to export mRNAs from the nucleus. In addition, TAP plays a central role in the nuclear export of (constitutive transport element) CTE containing viral mRNA. However, the recruitment of TAP to the two types of mRNA occurs by different mechanisms. TAP binds to the viral CTE directly, while the interaction with cellular mRNA is found to be indirectly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Multipotent Genetic Suppression of Retrotransposon-Induced Mutations by Nxf1 through Fine-Tuning of Alternative Splicing
Concepcion D
PLoS Genetics, 5 (2009)
Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex
Kataoka N, et al.
The Embo Journal, 20(22), 6424-6433 (2001)
Menghan Mei et al.
Proceedings of the National Academy of Sciences of the United States of America, 121(22), e2314166121-e2314166121 (2024-05-20)
The nonstructural protein 1 (Nsp1) of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a virulence factor that targets multiple cellular pathways to inhibit host gene expression and antiviral response. However, the underlying mechanisms of the various Nsp1-mediated functions and
Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA
Bartels H and Luban J
Retrovirology (2014)
De novo triplication of 11q12.3 in a patient with developmental delay and distinctive facial features
Yamamoto T
Molecular Cytogenetics, 6 (2013)

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