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RAB0397

Sigma-Aldrich

Human PDGF-BB ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

PDGF subunit B, PDGF-2, Platelet-derived growth factor subunit B

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.549-400 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PDGFB(5155)

General description

The Human PDGF-BB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF-BB in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human PDGF-BB

Application

For research use only. Not for use in diagnostic procedures.
Human PDGF-BB ELISA Kit has been used to measure PDGF-BB (platelet-derived growth factor BB) concentration in human coronary artery smooth muscle cells (HCASMCs).

Biochem/physiol Actions

PDGF-BB (platelet-derived growth factor BB) stimulates lymphatic metastasis and intratumoral lymphangiogenesis. PDGF-BB might be implicated in regulation of angiogenesis and endothelial proliferation. PDGF-BB expressed in placental endothelial cell activates signal transducer and activator of transcription 3 (STAT3) pathway and recruits human placental multipotent mesenchymal stromal cells (hPMSCs) for placental vascularization. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Role of Myoendothelial Gap Junctions in the Regulation of Human Coronary Artery Smooth Muscle Cell Differentiation by Laminar Shear Stress.
Zhang Z
Cellular Physiology and Biochemistry, 39(2), 423-437 (2016)
Callie Frey et al.
Platelets, 31(7), 952-959 (2020-01-15)
Patients with heart failure (HF) and left ventricular assist devices (LVAD) have dysregulated thrombo-inflammatory responses, mediated in part by platelets. While studies of platelet activation have been undertaken in HF, changes in the platelet transcriptome in HF patients following mechanical
PDGF-BB induces intratumoral lymphangiogenesis and promotes lymphatic metastasis.
Cao R
Cancer Cell, 6(4), 333-345 (2004)
Shilpa Tiwari-Heckler et al.
Cell reports, 37(4), 109897-109897 (2021-10-28)
Elevated circulating activity of adenosine deaminase 2 (ADA2) is associated with liver fibrosis in nonalcoholic fatty liver disease (NAFLD). In the liver of NAFLD patients, ADA2-positive portal macrophages are significantly associated with the degree of liver fibrosis. These liver macrophages
PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.
Battegay EJ
The Journal of Cell Biology, 125(4), 917-928 (1994)
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