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Key Documents

P0101

Sigma-Aldrich

Anti-Profilin 2 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PFN2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~14 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

technique(s)

western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PFN2(5217)
mouse ... Pfn2(18645)
rat ... Pfn2(81531)

General description

Profilins are actin-monomer-binding proteins, abundantly expressed in all eukaryotic cells from yeast to mammals. Profilin 2 (PFN2) is the neuronal specific isoform. It is an actin-binding protein and is a member of the small G-actin-binding class of protein.

Immunogen

synthetic peptide corresponding to amion acid residues 128-140 of human profilin 2 conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-Profilin 2 (C-terminal) antibody produced in rabbit has been used in immunostaining and immunoblotting.

Biochem/physiol Actions

Profilins are key regulators of actin dynamics. Profilin 2 (PFN2) associates with a number of proteins involved in membrane trafficking and endocytosis, such as synapsin and dynamin 1. It associates with dynamin 1 through the C-terminal proline-rich domain of dynamin 1 and thereby competes with the binding of SH3 ligands, such as endophilin and amphiphysin, thus interfering with the assembly of the endocytic machinery. Overexpression of profilin 2 inhibits endocytosis. Absence of profilin 2 in neurons increases endocytosis and membrane recycling. Profilin 2 is required for stimulated actin polymerization in the synapse and is involved in the regulation of synaptic vesicle exocytosis, neuronal excitability and complex behavior.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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