Yes, Neomycin is commonly used to select mammalian cells that have been successfully transfected with the Neomycin resistance gene (NeoR/neo).
N1142
Neomycin
solution, suitable for cell culture, BioReagent
Synonym(s):
Antibiotic 10676, Neomycin B
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Product Name
Neomycin solution, with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture
biological source
Streptomyces fradiae
sterility
0.1 μm filtered
product line
BioReagent
form
liquid
potency
8-12 mg per mL
packaging
pkg of 20ML
concentration
10 mg/mL in 0.9% NaCl (neomycin)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
clear
UniProt accession no.
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
Mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]1O
InChI
1S/C23H46N6O13/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22/h5-23,30-36H,1-4,24-29H2/t5-,6+,7-,8+,9-,10-,11-,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+/m1/s1
InChI key
PGBHMTALBVVCIT-VCIWKGPPSA-N
Gene Information
human ... TERT(7015)
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General description
Chemical structure: aminoglycoside
Application
Biochem/physiol Actions
Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B.[4] It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria
Caution
This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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