Films are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized and then stained for Gram-negative organisms with safranin O. For delineating Gram-positive and Gram-negative organisms in films.
Yersinia pestis is the causative agent of plague. Previously we have isolated an attenuated Y. pestis transposon insertion mutant in which the pcm gene was disrupted. In the present study, we investigated the expression and the role of pcm locus
We have previously shown that the cell morphogenesis NlpD lipoprotein is essential for virulence of the plague bacteria, Yersinia pestis. To elucidate the role of NlpD in Y. pestis pathogenicity, we conducted a whole-genome comparative transcriptome analysis of the wild-type
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