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H4416

Sigma-Aldrich

HypoThermosol® FRS Preservation Solution

Synonym(s):

hypothermic preservation medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

Application

HypoThermosol is an optimized hypothermic (2-8°C) preservation media that enables improved and extended preservation of cells, tissues and organs. HypoThermosol-FRS is uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process.

HypoThermosol-FRS includes key ions at concentrations that balance the intracellular state at hypothermic temperatures. Additional components include pH buffers, energy substrates, free radical scavengers, and osmotic/oncotic stabilizers.

Preparation Note

Usage and Preservation Protocol

  • Wipe down outside of container with 70% ethanol before opening.
  • Contents are sterile. If seal has been broken, do not use and contact BioLife Solutions.
  • HypoThermosol FRS is ready to use. Simply replace culture medium with chilled (<10°C) HypoThermosol FRS and maintain at 2-8°C.
  • At the end of the cold storage period, simply remove samples from the cold, decant the cold HypoThermosol FRS and replace with warm (20-37°C) culture medium of choice.

HypoThermosol FRS is not designed as a cell culture medium and should not be used above 15°C or warmed to 37°C

Legal Information

HypoThermosol is a registered trademark of BioLife Solutions, Inc.

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Description
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Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Alina Ostrowska et al.
Archives of toxicology, 83(5), 493-502 (2009-03-20)
Until now little is known about the functional integrity of human hepatocytes after hypothermic storage. In order to address this limitation, we evaluated several commercially available hypothermic preservation media for their abilities to protect freshly isolated hepatocytes during prolonged cold

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