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G2150

Sigma-Aldrich

L-Glutamine solution

200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

Synonym(s):

L-Glutamine for cell culture

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About This Item

CAS Number:
56-85-9
MDL number:
MFCD00008044
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24895088
NACRES:
NA.75

grade

Hybri-Max™

sterility

sterile-filtered

Assay

≥99% (TLC)

form

solution

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

color

clear colorless

shipped in

dry ice

storage temp.

−20°C

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

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General description

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Application

The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.

Preparation Note

Prepared in cell culture grade water.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of human antibodies from transgenic mice.
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Amyloid β-protein (Aβ) toxicity is hypothesized to play a seminal role in Alzheimer's disease (AD) pathogenesis. However, it remains unclear how Aβ causes synaptic dysfunction and synapse loss. We hypothesize that one mechanism of Aβ-induced synaptic injury is related to
Glutamine and antioxidants in critically ill patients.
Daren Heyland et al.
The New England journal of medicine, 369(5), 484-485 (2013-08-02)
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Chromatograms

application for HPLC

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