G1544

Anti-GFP, N-terminal antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Green Fluorescent Protein
• MDL number: MFCD05665217
• UNSPSC Code: 12352203
• NACRES: NA.56

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates; western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Specificity

Specific for GFP fusion proteins.

Immunogen

synthetic peptide corresponding amino acids 3-17 of GFP.

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:

• in immunoblotting
• in the histochemistry and immunohistochemistry
• in the immunoprecipitation

Biochem/physiol Actions

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves