D9281
Lambda DNA EcoR I Hind III Digest
for DNA electrophoresis
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About This Item
UNSPSC Code:
41141827
NACRES:
NA.25
Recommended Products
form
liquid
usage
100 uses (0.1 mg size)
500 uses (0.5 mg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Components
Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Other Notes
Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Preparation Note
Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.
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Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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