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CGP1

Sigma-Aldrich

Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

Cellular glutathione peroxidase, c-GPx

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

Quality Level

200

usage

sufficient for 100 tests

shelf life

Kit is stable for 24 months when unopened.

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

General description

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Suitability

Suitable for the measureent of glutathione peroxidase in tissue extracts

Principle

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Other Notes

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Kit Components Only

Product No.
Description
  • tert-Butyl hydroperoxide 1 mL

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

5.2 - Organic peroxides and self-reacting hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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