BHK 21 (Clone 13) from hamster
85011433, hamster kidney, Fibroblast
Synonym(s):
BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells
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About This Item
UNSPSC Code:
41106514
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product name
BHK 21 (Clone 13) from hamster, 85011433, from hamster kidney
biological source
hamster kidney
growth mode
Adherent
karyotype
2n = 44, pseudodiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Syrian kidney
Cell Line Description
Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.
Application
BHK 21 (Clone 13) from hamster has been used in viral transfection studies.
Virus replication studies
Culture Medium
GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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